| Classification |
Test name
(Click for details) |
| Validation study |
Peer reviews by experts |
OECD guidelines for the testing of chemicals |
Regulatory acceptance by, e.g., JaCVAM Regulatory acceptance board |
Recommendation to government |
|
Collaboration |
| Corrosivity test |
(1) Reconstructed human tissue (developed in Japan; Vitrolife-Skin) cytotoxicity assay |
| NIHS |
JaCVAM |
OECD TG No.431 |
Jun-08 |
Aug-08 |
|
Grants-in-Aid for Scientific Research from MHLW, JSAAE
|
| Phototoxicity test |
(1) 3T3 NRU (neutral red uptake) phototoxycity test |
| COLIPA and ECVAM |
NIHS |
OECD TG No.432 |
|
|
|
Grants-in-Aid for Scientific Research from MHLW, JSAAE |
| (2) Yeast growth inhibition phototoxicity assay and the red blood cell photohemolysis assay
|
| NIHS |
JaCVAM |
|
Jan-10 |
pending |
|
Grants-in-Aid for Scientific Research from MHLW, JSAAE |
| (3) ROS (reactive oxygen species) assay
|
|
Grants-in-Aid for Scientific Research from MHLW, JPMA |
| Skin sensitization test |
(1) Local lymph node assay (LLNA) |
| to be validated |
ICCVAM |
OECD TG No. 429 |
|
|
|
ICCVAM, ECVAM |
| (2) Reduced LLNA (rLLNA) |
| ECVAM and ICCVAM |
ICCVAM |
Updated OECD TG No. 429 |
Jul-12 |
Dec-12 |
|
ICCVAM、ECVAM, JaCVAM |
| (3) LLNA-DA (modified LLNA of Daicel based on ATP content)
|
| JaCVAM |
JaCVAM or ICCVAM |
OECD TG No. 442A |
Oct-12 |
Dec-12 |
|
Grants-in-Aid for Scientific Research from MHLW, JSAAE |
| (4) LLNA-BrdU (BrdU uptake murine LLNA)
|
| JaCVAM |
JaCVAM or ICCVAM |
OECD TG
No. 442B |
Oct-12 |
Dec-12 |
|
Grants-in-Aid for Scientific Research from MHLW, JSAAE |
| (5) Human cell line activation test (h-CLAT) for skin sensitization screening |
|
Grants-in-Aid for Scientific Research from MHLW, ECVAM |
| (6) Direct peptide reactivity assay (DPRA) |
|
ECVAM |
| (7) KeratinoSense assay |
|
ECVAM |
| (8) IL-8 Luc assay |
|
ICATM |
| Skin irritation test |
(1) Reconstructed human tissue (EPISKIN) MTT and IL-1 α assay |
| ECVAM |
ESAC |
not adopted as TG |
Jan-10 Mar-10 |
|
|
ECVAM |
| (2) Reconstructed human tissue (developed in Japan; LabCyte) assay
|
| JaCVAM |
OECD |
OECD TG
No. 439 |
under examination |
|
|
Grants-in-Aid for Scientific Research from MHLW, JSAAE |
| (3) Reconstructed human tissue (EPISKIN, EpiDerm, SkiEthic) MTT assay |
| ECVAM |
ESAC |
OECD TG No. 439 |
Jul-12 |
Dec-12 |
|
ECVAM |
| Skin absorption test |
In vitro assay |
|
|
| Ocular irritation test |
(1) Bovine corneal opacity and permeability (BCOP) assay
|
| ECVAM and ICCVAM |
ECVAM and ICCVAM |
OECD TG No. 437 |
09 / on going |
Dec-09 |
|
ICCVAM |
| (2) Isolated chicken eye (ICE) assay |
| ECVAM and ICCVAM |
ECVAM and ICCVAM |
OECD TG No. 438 |
09 / on going |
Dec-09 |
|
ECVAM |
| (3) Fluorescein leakage test |
|
ECVAM |
| (4) Cytosensor microphysiometer test |
| finished in ECVAM |
ESAC |
TG draft proposed |
under examination |
|
|
ECVAM |
| (5) Cytotoxicity test: short time exposure (STE) asssay
|
|
Grants-in-Aid for Scientific Research from MHLW, JSAAE |
| (6) Cytyotoxicity test: the rabbit cornea-derived cell line (SIRC-CVS) assay |
|
Grants-in-Aid for Scientific Research from MHLW
ICATM |
| (7) Reconstructed human tissue (developed in Japan; MATREX) cytotoxicity assay |
|
|
| (8) Reconstructed coreal tissue assay |
|
ECVAM |
| (9) Collagen gel (Vitrigel)-eye irritation test (EIT) |
|
MAFF
ICATM |
| (10) Eye irritation assay using anesthetics |
|
ICCVAM |
| Genotoxicity test |
(1) In vitro mammalian cell micronucleus test （chromosome abnormality test) |
| |
|
Updated OECD TG No. 473 and 487 |
on going |
|
|
JEMS |
| (2) In vitro commet assay |
|
Grants-in-Aid for Scientific Research from MHLW, MMS group in JEMS, ICCVAM, ECVAM |
| (3) In vivo comet assay |
|
Grants-in-Aid for Scientific Research from MHLW, MMS group in JEMS, ICCVAM, ECVAM |
| (4) Reconstructed skin tissue micronucleus test |
|
ECVAM |
| Acute oral toxicity test |
(1) In vitro assay |
| ECVAM and ICCVAM |
ICCVAM |
OECD GD No. 129 |
Apr-11 |
Jun-11 |
|
ICCVAM
ECVAM |
| Endcrine disrupter screening |
(1) Estrogen receptor-stably transfected transactivation assay (ER-STTA) for the screening of agonistic activitiy of chemicals |
| METI |
OECD |
OECD TG No. 455 |
|
|
|
OECD, METI |
| (2) ER-STTA for the screening of anti-agonistic activity of chemicals |
|
Grants-in-Aid for Scientific Research from MHLW, METI, KFDA, ECVAM |
| (3) BG1Luc estrogen receptor transactivation assay (ERTA) for the screeing of agonistic and anti-agonistic activity of chemicals
|
| ICVAM |
ICVAM |
OECD TG No. 457 |
under examination |
|
|
Grants-in-Aid for Scientific Research from MHLW, NICEATM, ICCVAM, ECVAM, JaCVAM |
| (4) CCi MCF-7 assay for the screening of estrogenic and anti-estrogenic activity of chemicals
|
|
ICCVAM, KoCVAM, JaCVAM |
| (5) MELN (derived from MCF-7) cell assay for the screening of estrgenic and anti-estrogenic activity of chemicals |
|
ECVAM |
| (6) AR-EcoScreen (the cell line expressing androgen receptor) assay for the screening of androgenic activity of chemicals |
|
METI |
| Teratogenetic activity prediction test |
(１) Hand1 (heart and neural crest derivatives expressed 1)-Luc (luciferase) assay
|
|
NEDO
METI
ICATM |
| Cell transformation assay for the screening of carcinogenitic activity of chemicals |
(1) Bhas 42 cell transformation assay |
|
NEDO
METI |
| (2) Syrian hamster embryo (SHE) cell transformation assay |
| ECVAM |
ESAC |
TG draft proposed |
on going |
|
|
ECVAM |
| (3) BALB/c 3T3 cell transformation assay |
|
ECVAM |
| Pyrogen screening test |
(1) In vitro assay
|
| ECVAM |
ECVAM or ICCVAM |
European Pharmacopoeia |
Dec-10 |
susupended |
|
ECVAM
ICCVAM |
